The purpose of this work was to develop a complex model of the conjugated link between biophysical and biochemical processes providing energy of protein and water molecules and ions during hydration and metabolism of muscle tissue eukaryotes.

Biological liquid containing proteins and ions can have electromagnetic properties and essentially differ from normal structured water. Hydrated ionic diameters being characterized by the size, weight and mobility determine the degree of hydration and development of eukaryotic energy. On this basis, the association-induction hypothesis was set up. It confirms that living protoplasm represents a specific three-dimensional lattice composed of protein, water and salts that can merge into a fixed charge system (4).

When investigating electromagnetic field (EMF) energy of eukaryotes, it should be noted that according to the mechanical Maxwell's model, the sum of all polarized "bound" and free charges can be equal to the striction forces, equivalent action of ponderomotor forces [5]. The latter can determine the degree of surface tension of the volume of deformed material medium and actively effect on cell hydration and dehydration.

Metabolism of proteins, fats and carbohydrates is based on the biochemical
Embden-Meierhof-Krebs and Warburg-Dickens-Lippmann cycles that finally produce the flow of protons. ATP and creatin phosphate maintain the muscle cell contractility to any agent of aggression and involve the muscle tissue in metabolism through a common non-specific reaction. Proteins achieve a high mobility and reactivity due to the presence of enzymes, hormones, water molecules and charges located along polypeptide amino acid chains (3). Biomacromolecules interchanging through a high proton rate enable an abrupt energy transfer to water molecules which accelerate the water flow in the cell protoplasm structure (2).

Muscles contain almost half of all body water that is investigated using the double fractionation model. Bound water in muscles amounts 5-10% of the free fraction and reflects its biophysical properties. The nuclear magnetic resonance (NMS)-spectroscopy allows to evaluate most objectively the relaxation dependence of water protons in magnetic fields due to rapid proton diffusion between water fractions (7).

The coupled reaction of the histological structure manifests itself as an active "switching on" of muscle cell organelles, increased nucleoli activity and pulsation, proportionally to the load, and locally decreased pH value. In extreme states, the complete ultrastructure of the muscle cell is alternately involved in work. An automatic connection of myosin fibers is a part of a specific link aimed at producing a mechanical impulse (force х time) and transferring kinetic energy to free protons ensuring their acceleration (6).

In the hypothesis presented by us earlier, the above mechanisms taken as whole implement the function of eukaryotes on the biophysical basis (1). The nuclear hydrogen complex (NHC) of muscles can contain: 1) Self-exciting EMF resulted from the functioning of the multilayer polarized muscle tissue structure; 2) Metabolic cyclic processes producing protons; 3) Active cell organelles responding to stress through their hypertrophy or hyperplasia, proportionally to the load; 4) Specific function of cell nuclei of myosin fibers and their cyclic involvement in proton activity, with energy transfer to water molecules.

Thus, the function of the muscle NHC can be based on the interaction of electromagnetic energy of the multilayer polarized structure of biological water and metabolism energy through cyclic biochemical reactions ensuring the production of protons. In extreme states, the activity of muscle cell NHC increases, which is confirmed by proton saturation of muscle mass in energetic active zones, reduction of the pH value and relaxation time of hydrogen nuclei in water, registered Т₁ and T₂ NMR-parameters.

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383 p

4. Ling G. Physical theory of the living cell: unnoticed revolution. SPb.: Nauka, 2008, 376 p

5. Tamm I.E. Foundations of electrical theory. Moscow: Nauka, 1976, 616 p

6. Dawson M.J., Gadian D.G., Wilkie D.R. Muscular fatigue investigated by phosphorus nuclear magnetic resonance. Nature, 1978, 274, 861-866

7. Foster K.R., Resing H.A., Garroway A.N. Science, 1976, 194, 4262,

324-326