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Home / Issues / № 2, 2013

Materials of the conference "EDUCATION AND SCIENCE WITHOUT BORDERS"

IMPACT ON THE CHARACTER CHITOSAN GEMATOMORFOLOGICAL PARAMETERS OF BLOOD SIMMENTAL COWS AUSTRIAN SELECTION
Tairova AR, Albulov A.I, Mukhamedyarova L.G.

Introduction

Change in living conditions, as well as any over-activation processes can lead to a shift in the parameters of the internal environment, so getting a viable organism, adapted to local conditions and having high economic value, is a serious problem of livestock [1]. There is an urgent need to study the patterns of adaptation of imported cows in the new environmental-economic conditions and find drugs that enhance their adaptive capacity. Today, one of the high-tech areas in the protection of animal health is to correct the conditions maladjustment using drugs of natural origin. Undoubted interest in this regard are based drugs chitin / chitosan [2].
This was the basis for the study of the influence of chitosan on blood parameters gematomorfologicheskie Simmental cows Austrian selection contained in the new environmental-economic conditions of the Southern Urals.

Materials and methods

The paper summarizes the results of scientific research on the health status and the adaptive capacity of the Austrian Simmental cattle breeding, imported into the economy of the Southern Urals on the program of the national project "Development of agriculture."

Assessment of adaptive capacity of the organism cows spent on morphological parameters in terms of blood and body condition of cows, calculated by the formula: PS = K (e + l + m / nL), where: K - normalizing factor equal to 102; e, l, m, n - number of eosinophils, lymphocytes, monocytes, neutrophils, red blood cells 105, respectively; L - number of leukocytes in blood ml3 1.
To study the adaptogenic effect of acid-soluble chitosan with a molecular mass of 120 kDa and a degree of deacetylation of 81% of us were divided into two groups of cows with 10 goals each, selected on the basis of pairs Analog: I group served as controls and received the basic diet of the economy; II group - experienced - in addition to the main received chitosan ration of 2 ml / kg of animal body weight once a day for two 5 - day course at intervals of 5 days. The material of the study were whole blood. Blood samples were taken from the cows in the morning before feeding, and then 10, 30 and 60 day studies.

Results and discussion.

The results of scientific and business experience have shown that chitosan causes characteristic changes in the red blood - and leykopoeza. Thus, at 30 and 60 days in cows II studies found a significant reduction of the amount of erythrocytes to 20.44 and 26,45% (p <0,01), increase in hemoglobin 34,84 and 39,17% (p <0 , 01), respectively, on days research and increase the color index to 0.57 (30 day) and 0.64 (60 days). Installed changes indicate activation of aerobic oxidation and tissue respiration in the body of the experimental group of cows with increasing buffer stocks of the organism.

Quantitative analysis of white blood cells when using chitosan cows showed a significant decrease in the number of neutrophils in 60 day studies, average 20% compared to control. The use of chitosan had a positive impact on the quantitative content of ± 0,11; 3,02 ± 0,005%), eosinophils (2,71 ± 0,004; 0,22 ±basophils (0,19 0,13%) and monocytes (3,21 ± 0,11; 4,42 ± 0,10%), respectively at 30 and 60 days of experiment. When you consider that monocytes are mononuclear leukocytes are involved in the production of immune bodies, it is likely that chitosan, showing immunotropnye properties, increases the body's defenses imported cows.
Changes in the state, which characterizes the degree of stress the body of cows reached 19,00 ± 0,53 for 30 days of the experiment and 27,00 ± 0,84 - by the end of the observation period, with 14,00 ± 0,29 - in control. indicates the intensity of the restructuring functional systems of cows and increase the adaptive capacity of the organism cows in the appointment of their chitosan.



References:
1. Letyagina, EN. Contact stress with milk production, the types of higher nervous activity and eating behavior in high yielding cows [Text]: Dis. ... Candidate. biol. Science / EN Letyagina. - Tyumen, 2004.-158 c.

2. Some areas of application of various forms of chitosan in veterinary practice [Text] / A. Albulov, M. Frolov, AY Samuylenko and others / / The current state and problems of animal welfare: Material. Intern. Conf. - Yalta, 2005. - C - 45 - 50.



Bibliographic reference

Tairova AR, Albulov A.I, Mukhamedyarova L.G. IMPACT ON THE CHARACTER CHITOSAN GEMATOMORFOLOGICAL PARAMETERS OF BLOOD SIMMENTAL COWS AUSTRIAN SELECTION. International Journal Of Applied And Fundamental Research. – 2013. – № 2 –
URL: www.science-sd.com/455-24467 (28.03.2024).