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Home / Issues / № 2, 2014

THE INFLUENCE OF DINITROSYL IRON COMPLEXES ON THE ALDEHYDE DEHYDROGENASE ACTIVITY OF THE RATS ORGANS DURIN THE BURN
Soloveva A.G.

Thermal injury is characterized by strengthening of catabolic processes, violation of the biosynthesis of proteins, decreased of some oxidoreductases activity, the development of the syndrome of endogenous intoxication [1]. It is known that nitric oxide participates in the regulation of many metabolic and physiological processes in the organism. The aim of the work was the study of influence of dinitrosyl iron complexes (DNIC, deposited form of NO) on the activity of aldehyde dehydrogenase (ALDH, the marker of biotransformation) in the liver, heart, lungs, kidneys of rats with thermal injury.

Material and methods. Experiments were conducted in rats of line Wistar. The combined thermal trauma (contact burn (20%, 7 sec.) and thermoinsulation injury (20 sec.) at animals was applied under anesthesia. The rats in the control group (n=10) daily were injected intraperitoneally 1 ml of physiological solution. The rats of experimental group (n=10) with the first day were spent infusion therapy with inclusion of an aqueous solution of dinitrosyl iron complexes (1 ml; 0,3 mmol/l). DNIC were received by the method of A.F. Vanina [2]. Rats were scored under anesthesia at 3 and 10 days after injury. ALDH activity was determined by method of B.M. Kerchengolts, L.P. Ilyina [3] in 10% homogenate of tissues (liver, kidney, heart and lungs).

Results and discussion. It is shown that in combined thermal injury specific activity of aldehyde dehydrogenase statistically significant decreases in lungs on the 3rd day after burn on 40%, on the 10 day after injury on 52% in comparison with the control animals. Enzyme activity significantly decreased only on the 3rd day after the defeat by 38% in comparison with healthy animals in the heart. Specific activity of ALDH in kidney is statistically significantly below the norm on the 3rd day after burn by 33% in comparison with the control group of animals. The reduction of aldehyde dehydrogenase activity is a poor prognostic sign, as it can lead to accumulation of highly toxic aldehydes. Aldehydes in elevated concentrations inhibit the activity of many enzymes, called intra - and intermolecular crosslinks of polypeptides, disturb the structure and function of cell membranes. The fall of the specific activity of aldehyde dehydrogenase in rats with thermal injury may be associated with increased content of highly toxic compounds in particular molecules average molecular weight. These molecules bind to the enzyme and transfer it to the new conformation state, which is characterized by low affinity of enzyme to the substrate of reaction [4].

In the liver were revealed compensatory increase of the specific activity of aldehyde dehydrogenase on the 10 days after thermal injury in 2,2 times in comparison with the control group of animals. The increase of enzyme activity, probably, can be explained by the onset of the third phase of endogenous intoxication and penetration of toxic compounds in the cells.

The introduction of nitric oxide deposited its form (DNIC) contributed to the increase of ALDH  activity in the lungs at 3 and 10 days after burn. In the heart under the influence of dinitrosyl iron complexes the activity of ALDH statistically significantly increased in 4,5 times on the 3rd day after the injury in comparison with the untreated animals and exceeded the indicator of healthy rats in 2,8 times. The normalizing effect of DNIC on the ALDH activity in the rat liver during the burn was identified: enzyme activity statistically significantly decreased by 10 days after the injury by 40% compared to the untreated animals. Under the influence of deposited form of nitric oxide in the kidneys the enzyme activity decreased.

Conclusion. Thus, burn trauma causes a decrease of the specific activity of aldehyde dehydrogenase in the lungs, heart and kidneys. It is shown that dinitrosyl iron complexes have a positive impact on the catalytic properties of ALDH in all investigated organs when combined thermal injury.



References:
1. Koziniec G.P. et al. Burn intoxication. Pathogenesis, clinic, treatment guidelines, 2005. – Moscow: MEDpress-Inform. – 321 p.

2. Shumaev K.B. et al. Interaction of dinitrosyl iron complexes with intermediates of oxidative stress // Biophysics. – 2006. – Vol. 51, №3. – Р.472-477.

3. Kerchengolts B.M., Ilyina L.P. Biological aspects of alcohol pathologies and addictions, 1998. – Yakutsk: Publishing YSU, 150p.

4. Zimin, Y.V., Soloveva A.G. The possible mechanism of action of the "termotoxins" on the kinetic properties of liver aldehyde dehydrogenase in experimental thermal injury // Bulletin of the Russian Academy of Military Medicine. – 2010. – Vol. 29, № 1. – P. 43-44.



Bibliographic reference

Soloveva A.G. THE INFLUENCE OF DINITROSYL IRON COMPLEXES ON THE ALDEHYDE DEHYDROGENASE ACTIVITY OF THE RATS ORGANS DURIN THE BURN. International Journal Of Applied And Fundamental Research. – 2014. – № 2 –
URL: www.science-sd.com/457-24573 (21.08.2019).