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Home / Issues / № 2, 2011

THE PROCESSES OF LIPID PEROXIDATION WITHIN THE PATIENTS WITH CHRONIC OBSTRUCTIVE LUNG DISEASE
Ganceva H.H., Kamilov F.H., Iliasova T.M., Gabitova D.M.

According to modern concept, the pathophysiological base of progressive obstruction of airways is a chronicle infl ammatory process in lungs, when in answer to the action of destructive factors of external environment there is breaking the balance between the activeness of pro- and antioxidant, proteoclastic and antiproteoclastic processes [5, 6, 7].

The aim of our research is studying of the level of products of lipid peroxidation and activeness of ferments of antioxidant protection (AOP) in the blood plasma and condensate of breathed out air within patients with COLD of different degree of severity at the phase of intensifi cation, as supplementary diagnostic criterions of course of infl ammatory process.

Materials and methods

In all there were examined 55 men, who suffered from COLD of average and severe degree of severity, the average age of which was 56,7 ± 4,6 years. The criteria of selection to the group of researched patients with COLD were the presence within them of volume of forced exhalation in 1 second (VFE1) < 80 %, VFE1/Maximal Expiratory Flow Volume < 70 %, lasting experience of smoking more than 20 years, at the average – 34,4 ± 1,8 years. The criteria of exception were: bronchial asthma, accompanying diseases at the stage of decompensation. From the whole number of patients in the I group there were included 28 patients with COLD of average degree of severity, the average age of which was 52,6 ± 2,8 years, experience of smoking – 29,2 ± 5,1 years, The index of smoking person (IS) – 200,4 ± 46,8, period of disease – 14,7 ± 2,5 years, volume of forced exhalation in 1 second (VFE1) was – 56,9 ± 3,9 %, maximal expiratory fl ow volume (MEFV) – 87,7 ± 3,4 %, VFE1/MEFV – 64,9 ± 4,9, maximal speed of Exhalation (MSE) – 357,8 ± 23,4 ml, average pressure in the lung arteries (APLA) – 27,4 ± 1,2 mm of mercury, carbonation of oxygen (SaО2) – 95,5 ± 0,31 %. Into the II group there were included 27 patients with COLD of severe degree of severity, the average age of which was 59,8 ± 1,7 years, experience of smoking – 38,6 ± 4,2 years, IS – 334,4 ± 24,6, period of disease – 19,4 ± 1,1 years, VFE1 – 31,1 ± 3,6 %, MEFV – 45,8 ± 4,7 %, VFE1/MEFV – 67,9 ± 6,4, MSE – 181,4 ± 13,68 ml, APLA – 38,3 ± 3,1 mm of mercury, SaО2 – 89,6 ± 1,18 %. The control group was made out of 27 practically healthy men of corresponding age.

The biochemical methods of research included defi nition in the blood plasma and CBA of initial products of lipid peroxidation – diene conjugates (DC), second – ketodienes (KD) and joint trienes (JT) by the method of Volchegorskyi and others [4], malondialdehyde (TBA – reactive product) by the method of Karpishenko A.I. [7], and also fi nal products of lipid peroxidation – Schiff basis (SB) by the method of Volchegorskyi I.A. and others [4]. The level of activeness of lipid peroxidation and peroxydase of plasma was estimated by the methods of Terekhina N.A. and Petrovich U.A. [6], catalase – by the methods of Koroluk M.A. and others [8]. The statistic treatment of material was carried out with the help of standard methods of varietive statistic of medico-biological profi le with the help of computer program «Statist».

Results and discussion.

Within examined patients with COLD of average and severe degree of severity at the phase of intensifi cation there were observed abnormalities at the system of lipid peroxydation – antioxidant protection, which was characterized by the presence of system oxidative stress, which is expressed by the considerable hyperproduction of free radical metabolites and increase of second and fi nal products of lipid peroxidation.

Free radical oxidation has one of the key role at the molecular mechanisms of pathogenesis of COLD [2, 3, 5, 6]. From this < there was carried out the comparative analysis of content of products of lipid peroxidation in the plasma and CBA within patients with COLD of I and II groups. While the analysis of average values of concentration of products of lipid peroxidation in the blood plasma and CBA within patients with COLD of I and II groups at the phase of intensifi cation there was observed statistically meaningful increase (р < 0,05) KD, JT, SO and TBA of products in comparison with control. With the increase of degree of severity of disease the intensiveness of processes of lipid peroxidation in the researched spheres increased. It was revealed, that within patients with COLD of severe degree at the phase of intensiveness the content of fi nal products of lipid peroxidation (SB) at the blood plasma and CBA is considerably higher (р < 0,05) in comparison with patients with COLD of average degree of severity.

Within researched patients there was studied the activeness of ferments of antioxidant protection (MSE, catalase, peroxidase) at the blood plasma and CBA. The analysis of average values of avtiveness of researched ferments showed, that within patients with COLD Of I and II group there occurs exhaustion of antioxidant protection in comparison with control, mainly at the late stages of disease. Within patients of II group at the blood plasma the activeness of peroxidase was decreased in comparison with patients of I group (р < 0,05).

Carried out researches confirm the increasing number of proves, that while the COLD there occurs disbalance at the system of oxidants-antioxidants to the side of oxidants [2, 3, 6]. Within observed patients with COLD of average and severe degree of severity at the stage of intensiveness the markers of oxidative stress were found at the blood plasma and condensate of breathed out air.

Conclusions

  1. Chronic obstructive lung disease at the phase of intensification is characterized by the strengthening of peroxidation of lipids and depression of fermentative link of antioxidant protection in the blood plasma and condensate of breathed out air, which are progressing by the measure of increase of the severity of disease.
  2. The change of the level of products of lipid peroxidation and activeness of ferments of antioxidant protection in the condensate of breathed out air within patients with COLD at the phase of intensification allows to use this non invasive method for the estimation of condition of system oxidants-antioxidants within this group of patients.

References

  1. Avdeev S.N. Cronicle obstructive disease of lungs as a systemic disease / S.N. Avdeev // Pulmonology. - 2007. - №7. - P. 104-116.
  2. Anaev E.H. Research of condensate of breathed out air in pulmonology (review of foreign literature) / E.H. Anaev, A.G. Chuchalin // Pulmonology. - 2002. - №2. - P. 57-64.
  3. Berezuk, L.V. The research of changes of lipid peroxidation within patients with COLD // Collection of works of the IV congress of pulmonologists of Ural federal district. - Chelyabinsk, 2004. - P. 6-8.
  4. Volchegorskyi I.A. Comparison of different approaches to the definition of products of lipid peroxidation in a heptane-isopropanol extracts of blood / I.A. Volchegorskyi, A.G. Nalimov, B.G. Yarovinskyi, R.I. Livshic // Questions of medical chemistry. - 1989. - №1. - P. 127-130.
  5. Global Initiative for Chronic Obstructive Lung Disease (GOLD) // National Heart, Lung, and Blood Institute. - Publication № 2701, April 2001.
  6. Global Initiative for Chronic Obstructive Lung Disease (GOLD). Global strategy for diagnosis, management, and prevention of chronic obstructive pulmonare disease. NHLBI/WHO workshop report. Last updated 2006.
  7. Karpishenko A.I. Medical laboratory technologies / A.I. Karpishenko. - St. Petersburg, 1999. - P. 618-647.
  8. Koroluk M.A. The method of definition of the activeness of catalase / M.A. Koroluk, L.I. Ivanova, I.G. Maiorova // Laboratorial work. - 1988. - №1. - P. 16-19.

The work was submitted to the International Scientific Conference «Modern science intensive technologies», Spain (Tenerife island), 20-27 of November 2010, сame to the editorial office 05.10.2010.


Bibliographic reference

Ganceva H.H., Kamilov F.H., Iliasova T.M., Gabitova D.M. THE PROCESSES OF LIPID PEROXIDATION WITHIN THE PATIENTS WITH CHRONIC OBSTRUCTIVE LUNG DISEASE. International Journal Of Applied And Fundamental Research. – 2011. – № 2 –
URL: www.science-sd.com/389-23518 (04.12.2021).