Material and methods. The experiments were conducted on 50 white Wistar rats weighing 160-180g. Animals were divided into 5 groups of 10 animals each: group 1 - control (healthy rats), group 2 - animals, who daily intraperitoneally injected 1 ml saline solution saturated with oxygen, 3, 4, 5 group - animals who have been treated with ozone. Ozone was obtained from the oxygen produced by the oxygen concentrator, with the help of a medical device "MEDOZONS SYSTEMS". The drug was administered to rats 3, 4, 5 groups daily 30 days intraperitoneally in the form of ozonated physiological solution in the amount of 1 ml with different ozone doses in him - 0.6 mcg/l, 2.0 mcg/l and 8.0 mcg/l at saturating concentrations of ozone in the ozone-oxygen mixture 3000 mcg/l, 10000 mcg/l, 40000 mcg/l respectively. The concentration of glucose and lactate was determined on the analyzer SUPER GL ambulance (Germany). The activity of lactate dehydrogenase (LDH) in direct reaction was evaluated by using as a substrate of lactic acid, in the reverse reaction using pyruvic acid [2]. The protein concentration was detected by the method of Lowry [1]. Statistical analysis was performed using the program Statistica 6.

Results and discussion. The molecular mechanisms of action of various drugs and biologically active compounds depend from the characteristics of enzymes regulation. There is no doubt that in this case LDH plays an important role in the regulation of energy metabolism of cells. In direct reaction pyruvate is formed from lactate. Pyruvate in subsequent under aerobic conditions can be used in the Krebs cycle. Reverse reaction of LDH leads to the formation lactate from pyruvate and characterizes the severity of the anaerobic process in the cell. It is shown that under the action of ozone at a dose of 3000 mcg/l LDH activity in direct response was increased by 66%, in a dose of 10000 mcg/l O3 – on 55%, at a dose of 40000 mcg/l – on 78% as compared with healthy animals. Increas of direct reaction activity of LDH typical for aerobic conditions of erythrocytes metabolism. The increased of LDH activity helps to reduce of lactate and accumulation of pyruvate. Therefore, under the influence of O3 is the activation intensity aerobic processes and metabolism in erythrocytes. The activity of lactate dehydrogenase in the reverse reaction statistically significantly increased when using an ozone dose of 3000 mcg/l to 58%, at the dose of 10000 mcg/l O3 – on 39%, at a dose of 40000 mcg/l by 34% compared to the control animals. The level of lactate in the blood statistically significantly decreased when using doses of ozone 3000 mcg/l, the dose of ozone 10000 mcg/l and 40000 mcg/l lactate concentration increased by 27% and 56%, respectively, compared with healthy animals. The level of glucose in the plasma under the action of ozone at a dose of 3000 mcg/l did not differ from the level of the substrate of the control animals, at the dose of 10000 mcg/l O3 concentration of glucose in plasma was increased by 90%, at a dose of 40000 mcg/l on 87% compared to the control animals. When using an ozone dose of 3000 mcg/l the glucose level in the erythrocytes decreased by 45% compared to control animals. Thus, the ozone at a concentration of 3000 mcg/l effect on carbohydrate metabolism increasing glycolysis, activation of glucose utilization by the tissues, reducing the level of glucose in the blood. This is achieved through stimulation of the pentose-phosphate shunt and aerobic glycolysis. Ozone doses 10000 and 40000 mcg/l caused an increase in the level of glucose in the erythrocytes by 47% and 54% compared with control.

Conclusion. 1. Thus, a dose-dependent effect of ozone on the metabolism of erythrocytes was identified. 2. Under the influence of ozone dose of 3000 mcg/l were marked positive changes in the concentrations of glucose and lactate in the blood, and lactate dehydrogenase activity in erythrocytes of animals.

2. Solovyоva A.G., Zimin Yu.V. A New Estimation Method of Blood Metabolism Dynamics of Patients with Heat Injuries. Modern Technologies in Medicine, 2012; 2: P. 116-117.